Biological identification of dust-associated microorganism in air duct systems

There are many varieties of dust-associated microbes. Identifies species from the dust-associated microorganism by selecting the air conditioning systems from three super markets in a city of Guangdong province in order to make sure the dominant microflora in sub-tropical areas between later spring and early summer. Extracts DNAs from separated bacteria and fungi, and uses 16S rRNA and ITS sequence to amplify the DNA fragments. Purifies the PCR products and performs sequencing reaction analyses. The results show that the four strains of bacteria are identified, i.e. Bacillus cereus, Staphylococcus epidermidis, Micrococcus luteus and Escherichia Coli, and the two strains of fungi, Penicillium decumbens and Aspergillus sydowii.